Applications of MicroArrays for Mass Spectrometry (MAMS) in Single-Cell Metabolomics
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Date
2020
Publication Type
Book Chapter
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yes
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Abstract
The metabolic network is the endpoint in the flow of information that begins with the "gene" and ends with "phenotype" (observable function) of the cell. Previously, due to the variety of metabolites analyzed inside cells, the metabolomic measurements were performed with samples including multiple cells. Unfortunately, this sampling process may mask important metabolic phenomena, such as cell-to-cell heterogeneity. For these studies, we must use analytical techniques that can robustly deliver reproducible results with single-cell sensitivity. In this chapter, we summarize laser-based methods for single-cell analysis and a novel approach of MicroArrays for Mass Spectrometry (or MAMS) is described in full detail. This particular type of microarrays was tailored for the study of cells grown in liquid medium using multiple-analytical readouts, such as optical and laser desorption/ionization (LDI) or MALDI mass spectrometry.
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published
Editor
Book title
Single Cell Metabolism
Journal / series
Volume
2064
Pages / Article No.
73 - 88
Publisher
Humana
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Edition / version
Methods
Software
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Subject
Single cell; Cell populations; Heterogeneity; Metabolites; Mass spectrometry; Laser; MALDI; Microarray; MAMS