Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems
OPEN ACCESS
Loading...
Author / Producer
Date
2022-05-23
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
OPEN ACCESS
Data
Abstract
From individual cells to whole organisms, O2 transport unfolds across micrometer- to millimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O2 dynamics via currently available methods difficult or unreliable. Here, we present “sensPIV,” a method to simultaneously measure O2 concentrations and flow fields. By tracking O2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, we measured O2 transport within (1) microfluidic devices, (2) sinking model aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliary movement to link zones of photosynthetic O2 production to zones of O2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
2 (5)
Pages / Article No.
100216
Publisher
Cell Press
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
imaging; oxygen; flow fields; nutrients; fluxes; corals; particles; aggregates; microfluidics