Modeling and measuring glucose consumption by cancer spheroids in hanging drops using integrated biosensors
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Date
2022-08
Publication Type
Conference Paper
ETH Bibliography
yes
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Abstract
As 3D in vitro tissue models become more pervasive, their built-in concentration gradients increase biological relevance at the cost of analysis simplicity. Investigating metabolism heterogeneity resulting from these gradients requires invasive methods and time-consuming analyses. An alternative is measuring concentrations around microtissues with biosensors. Our hanging-drop-integrated enzymatic glucose biosensors electrochemically measure analyte concentration within hanging-drop compartments hosting single cancer spheroids. We developed an analytical model correlating measured sensor currents and glucose concentrations. Our biosensors' low limit of detection, combined with our current-fitting method, allowed us to infer glucose distributions within cancer spheroids, which will help to translate in vitro tissue results to in vivo.
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Publication status
published
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Editor
Book title
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
Journal / series
Volume
3
Pages / Article No.
1455 - 1456
Publisher
Curran
Event
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Cancer spheroids; Glucose biosensor; Hanging drops; Mathematical modeling
Organisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas
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