Assembly and nuclear export of pre-ribosomal particles in budding yeast


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Date

2014-08

Publication Type

Journal Article

ETH Bibliography

yes

Citations

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Data

Abstract

The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that associate with pre-ribosomal particles at distinct stages to perform specific assembly steps. On their way through the nucleus, diverse energy-consuming enzymes are thought to release assembly factors from maturing pre-ribosomal particles after accomplishing their task(s). Subsequently, recruitment of export factors prepares pre-ribosomal particles for transport through nuclear pore complexes. Pre-ribosomes are exported into the cytoplasm in a functionally inactive state, where they undergo final maturation before initiating translation. Accumulating evidence indicates a tight coupling between nuclear export, cytoplasmic maturation, and final proofreading of the ribosome. In this review, we summarize our current understanding of nuclear export of pre-ribosomal subunits and cytoplasmic maturation steps that render pre-ribosomal subunits translation-competent.

Publication status

published

Editor

Book title

Journal / series

Volume

123 (4)

Pages / Article No.

327 - 344

Publisher

Springer

Event

Edition / version

Methods

Software

Geographic location

Date collected

Date created

Subject

Nuclear export; Assembly Factor; Nuclear pore complex; Nuclear Export Signal; Ribosome assembly

Organisational unit

03884 - Panse, Vikram G. (SNF-Professur) (ehem.) check_circle

Notes

It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.

Funding

260676 - Dissecting the biogenesis of eukaryotic ribosomal subunits (EC)

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