Synthetic microbiota reveal priority effects and keystone strains in the Arabidopsis phyllosphere


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Date

2019-10

Publication Type

Journal Article

ETH Bibliography

yes

Citations

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Data

Abstract

Multicellular organisms, including plants, are colonized by microorganisms, some of which are beneficial to growth and health. The assembly rules for establishing plant microbiota are not well understood, and neither is the extent to which their members interact. We conducted drop-out and late introduction experiments by inoculating Arabidopsis thaliana with synthetic communities from a resource of 62 native bacterial strains to test how arrival order shapes community structure. As a read-out we tracked the relative abundance of all strains in the phyllosphere of individual plants. Our results showed that community assembly is historically contingent and subject to priority effects. Missing strains could, to various degrees, invade an already established microbiota, which was itself resistant and remained largely unaffected by latecomers. Additionally, our results indicate that individual strains of Proteobacteria (Sphingomonas, Rhizobium) and Actinobacteria (Microbacterium, Rhodococcus) have the greatest potential to affect community structure as keystone species.

Publication status

published

Editor

Book title

Volume

3 (10)

Pages / Article No.

1445 - 1454

Publisher

Springer

Event

Edition / version

Methods

Software

Geographic location

Date collected

Date created

Subject

Bacteria; Microbial communities; Microbial ecology

Organisational unit

09583 - Sunagawa, Shinichi / Sunagawa, Shinichi check_circle
03740 - Vorholt, Julia / Vorholt, Julia check_circle

Notes

It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.

Funding

668991 - Structure function relationships of the phyllosphere microbiota (EC)

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