Bandwidth Compensation for High Resolution Impedance Spectroscopy
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Author / Producer
Date
2011
Publication Type
Conference Paper
ETH Bibliography
yes
Citations
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Abstract
This paper reports on a microfluidic differential impedance cytometer, which uses a bandwidth compensation technique together with a small detection volume and multi-frequency analysis to achieve an increased sensitivity. The bandwidth compensation technique allows for measurements within small bandwidths by accounting for the increased signal amplitude dependence on the particle speed. We demonstrate detection and clear baseline discrimination of polystyrene beads with diameters of 1 μm and 2 μm and the discrimination of 5 μm beads from yeast cells of similar size. We show that using multiple frequencies in parallel significantly improves the discrimination performance of the cytometer.
Permanent link
Publication status
published
External links
Book title
Eurosensors XXV
Journal / series
Volume
25
Pages / Article No.
1209 - 1212
Publisher
Elsevier
Event
25th Eurosensors Conference
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Impedance measurement; Flow cytometry; Single-cell analysis
Organisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas