Imaging samples larger than the field of view: The SLS experience
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Date
2017
Publication Type
Conference Paper
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yes
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Abstract
Volumetric datasets with micrometer spatial and sub-second temporal resolutions are nowadays routinely acquired using synchrotron X-ray tomographic microscopy (SRXTM). Although SRXTM technology allows the examination of multiple samples with short scan times, many specimens are larger than the field-of-view (FOV) provided by the detector. The extension of the FOV in the direction perpendicular to the rotation axis remains non-trivial. We present a method that can efficiently increase the FOV merging volumetric datasets obtained by region-of-interest tomographies in different 3D positions of the sample with a minimal amount of artefacts and with the ability to handle large amounts of data. The method has been successfully applied for the three-dimensional imaging of a small number of mouse lung acini of intact animals, where pixel sizes down to the micrometer range and short exposure times are required.
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published
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Journal / series
Volume
849
Pages / Article No.
12004
Publisher
IOP Publishing
Event
X-Ray Microscopy Conference 2016 (XRM 2016)
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Organisational unit
03817 - Stampanoni, Marco F.M. / Stampanoni, Marco F.M.
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Funding
135550 - In-vivo study of lung physiology with sub-second X-ray tomographic microscopy (SNF)