High-Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates
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Date
2023-01-04
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Programmable coacervates based on zwitterionic polymers are designed as dynamic materials for ion exchange bioseparation. These coacervates are proposed as promising materials for the purification of soft nanoparticles such as liposomes and extracellular vesicles (EVs). It is shown that the stimulus-responsiveness of the coacervates and the recruitment of desired molecules can be independently programmed by polymer design. Moreover, the polymeric coacervates can recruit and release intact liposomes, human EVs, and nanoalgosomes in high yields and separate vesicles from different types of impurities, including proteins and nucleic acids. This approach combines the speed and simplicity of precipitation methods and the programmability of chromatography with the gentleness of aqueous two-phase separation, thereby guaranteeing product stability. This material represents a promising alternative for providing a low-shear, gentle, and selective purification method for EVs.
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Publication status
published
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Book title
Journal / series
Volume
19 (1)
Pages / Article No.
2204736
Publisher
Wiley-VCH
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
coacervation; exosomes; ion exchange; liquid-liquid phase separation; purification; separation; vesicles
Organisational unit
09572 - Arosio, Paolo / Arosio, Paolo
Notes
Funding
801338 - Extracellular vesicles from a natural source for tailor-made nanomaterials (EC)
952183 - Biogenic Organotropic Wetsuits (EC)
952183 - Biogenic Organotropic Wetsuits (EC)