Enrichment of Persister Cells Through Β-Lactam-Induced Filamentation and Size Separation
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Author / Producer
Date
2021
Publication Type
Book Chapter
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no
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Abstract
Analyzing persisters at the single-cell level is crucial to properly define their phenotypic traits. However, single-cell analyses are challenging due to the rare and temporary nature of persister cells, thus requiring their rapid and efficient enrichment in a culture. Existing methods to isolate persisters from a bacterial population show important shortcomings, including contamination with susceptible cells and/or cell debris, which complicate subsequent microscopic analyses. We here describe a protocol to enrich persisters in a culture using β-lactam-induced filamentation followed by size separation. This protocol minimizes the amount of cell debris in the final sample, facilitating single-cell studies of persister cells.
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Publication status
published
Book title
Bacterial Persistence
Journal / series
Volume
2357
Pages / Article No.
63 - 69
Publisher
Humana
Event
Edition / version
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Date collected
Date created
Subject
Persister; Enrichment; Single-cell
Organisational unit
09490 - Stadler, Tanja / Stadler, Tanja
02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.