A Spectrofluorometric Method for Real-Time Graft Assessment and Patient Monitoring
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Date
2023-08-15
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Biomarkers are powerful clinical diagnostics and predictors of patient outcome. However, robust measurements often require time and expensive laboratory equipment, which is insufficient to track rapid changes and limits direct use in the operating room. Here, this study presents a portable spectrophotometric device for continuous real-time measurements of fluorescent and non-fluorescent biomarkers at the point of care. This study measures the mitochondrial damage biomarker flavin mononucleotide (FMN) in 26 extended criteria human liver grafts undergoing hypothermic oxygenated perfusion to guide clinical graft assessment. Real-time data identified seven organs unsuitable for transplant that are discarded. The remaining grafts are transplanted and FMN values correlated with post-transplant indicators of liver function and patient recovery. Further, this study shows how this device can be used to monitor dialysis patients by measuring creatinine in real-time. Our approach provides a simple method to monitor biomarkers directly within biological fluids to improve organ assessment, patient care, and biomarker discovery.
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Publication status
published
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Book title
Journal / series
Volume
10 (23)
Pages / Article No.
2301537
Publisher
Wiley-VCH
Event
Edition / version
Methods
Software
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Date collected
Date created
Subject
biomarkers; biomedical engineering; biosensors; liver transplantation; translational research
Organisational unit
09472 - Tibbitt, Mark / Tibbitt, Mark
Notes
Funding
ETH-24 21-1 - Lipid nanoparticle-mediated mRNA delivery for ex situ liver regeneration (ETHZ)