Design and construction of synthetic gene networks in mammalian cells
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Author / Producer
Date
2012
Publication Type
Book Chapter
ETH Bibliography
yes
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Abstract
Advances in the development of molecular tools for the inducible control of transcription, translation, and protein degradation are the basis for the rapidly emerging design and construction of synthetic gene net works in mammalian cells. In this chapter, we describe such tools and how they can be integrated into a synthetic gene network with desired functionality. The network design and construction process is illustrated in the form of a detailed protocol for the implementation of a logic NOR gate based on an inducible promoter combined with an inducible protein degradation system.
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Publication status
published
Book title
Synthetic Gene Networks
Journal / series
Volume
813
Pages / Article No.
359 - 376
Publisher
Humana Press
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Auxin; Boolean logic; Degron; Inducible expression; NOR gate
Organisational unit
03694 - Fussenegger, Martin / Fussenegger, Martin
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