A Synthetic Dynamic Polyvinyl Alcohol Photoresin for Fast Volumetric Bioprinting of Functional Ultrasoft Hydrogel Constructs
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Date
2023-05-12
Publication Type
Journal Article
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yes
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Abstract
Tomographic volumetric bioprinting (VBP) enables fast photofabrication of cell-laden hydrogel constructs in one step, addressing the limitations of conventional layer-by-layer additive manufacturing. However, existing biomaterials that fulfill the physicochemical requirements of VBP are limited to gelatin-based photoresins of high polymer concentrations. The printed microenvironments are predominantly static and stiff, lacking sufficient capacity to support 3D cell growth. Here a dynamic resin based on thiol-ene photo-clickable polyvinyl alcohol (PVA) and thermo-sensitive sacrificial gelatin for fast VBP of functional ultrasoft cell-laden hydrogel constructs within 7-15 s is reported. Using gelatin allows VBP of permissive hydrogels with low PVA contents of 1.5%, providing a stress-relaxing environment for fast cell spreading, 3D osteogenic differentiation of embedded human mesenchymal stem cells and matrix mineralization. Additionally, site-specific immobilization of molecules-of-interest inside a PVA hydrogel is achieved by 3D tomographic thiol-ene photopatterning. This technique may enable spatiotemporal control of cell-material interactions and guides in vitro tissue formation using programmed cell-friendly light. Altogether, this study introduces a synthetic dynamic photoresin enabling fast VBP of functional ultrasoft hydrogel constructs with well-defined physicochemical properties and high efficiency.
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published
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Journal / series
Volume
33 (20)
Pages / Article No.
2214393
Publisher
Wiley-VCH
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Date collected
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Subject
bioresins; hydrogels; polyvinyl alcohol; thiol-ene reactions; volumetric bioprinting
Organisational unit
03565 - Müller, Ralph / Müller, Ralph
09830 - Qin, Xiao-Hua / Qin, Xiao-Hua
Notes
Funding
188522 - Subtractive 3D Micro-Printing of Functional Osteocyte Networks as An In Vitro Model for Bone Organoids (SNF)
190345 - Mini-Bone-on-a-Chip: Microfluidic Engineering of 3D Osteocyte Networks in Void-forming Hydrogels (SNF)
190345 - Mini-Bone-on-a-Chip: Microfluidic Engineering of 3D Osteocyte Networks in Void-forming Hydrogels (SNF)