Web-based analysis of the mouse transcriptome using Genevestigator


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Date

2006-06

Publication Type

Journal Article

ETH Bibliography

yes

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Abstract

Background Gene function analysis often requires a complex and laborious sequence of laboratory and computer-based experiments. Choosing an effective experimental design generally results from hypotheses derived from prior knowledge or experimentation. Knowledge obtained from meta-analyzing compendia of expression data with annotation libraries can provide significant clues in understanding gene and network function, resulting in better hypotheses that can be tested in the laboratory. Description Genevestigator is a microarray database and analysis system allowing context-driven queries. Simple but powerful tools allow biologists with little computational background to retrieve information about when, where and how genes are expressed. We manually curated and quality-controlled 3110 mouse Affymetrix arrays from public repositories. Data queries can be run against an annotation library comprising 160 anatomy categories, 12 developmental stage groups, 80 stimuli, and 182 genetic backgrounds or modifications. The quality of results obtained through Genevestigator is illustrated by a number of biological scenarios that are substantiated by other types of experimentation in the literature. Conclusion The Genevestigator-Mouse database effectively provides biologically meaningful results and can be accessed at https://www.genevestigator.ethz.ch.

Publication status

published

Editor

Book title

Volume

7

Pages / Article No.

311

Publisher

BioMed Central

Event

Edition / version

Methods

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Subject

DNA-MIKROARRAY TECHNOLOGIE; DATENBEHANDLUNG + DATENAUSWERTUNG (EXPERIMENTELLE BIOLOGIE); GENEXPRESSION (GENETIK); MURIDAE (ZOOLOGIE); DNA MICROARRAYS; DATA TREATMENT + DATA INTERPRETATION (EXPERIMENTAL BIOLOGY); GENE EXPRESSION (GENETICS); MURIDAE (ZOOLOGY); Sirt1 Expression; Public Repository; Mutant Surveyor; Response Viewer; Edinburgh Mouse Atlas Project

Organisational unit

03340 - Widmayer, Peter (emeritus) / Widmayer, Peter (emeritus) check_circle
03554 - Gruissem, Wilhelm (emeritus) / Gruissem, Wilhelm (emeritus) check_circle
02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences

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