Model-based correction of rapid thermal confounds in fluorescence neuroimaging of targeted perturbation
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Date
2024-02
Publication Type
Journal Article
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yes
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Abstract
Significance: An array of techniques for targeted neuromodulation is emerging, with high potential in brain research and therapy. Calcium imaging or other forms of functional fluorescence imaging are central solutions for monitoring cortical neural responses to targeted neuromodulation, but often are confounded by thermal effects that are inter-mixed with neural responses. Aim: Here, we develop and demonstrate a method for effectively suppressing fluorescent thermal transients from calcium responses. Approach: We use high precision phased-Array 3 MHz focused ultrasound delivery integrated with fiberscope-based widefield fluorescence to monitor cortex-wide calcium changes. Our approach for detecting the neural activation first takes advantage of the high inter-hemispheric correlation of resting state Ca2 dynamics and then removes the ultrasound-induced thermal effect by subtracting its simulated spatio temporal signature from the processed profile. Results: The focused 350 μm-sized ultrasound stimulus triggered rapid localized activation events dominated by transient thermal responses produced by ultrasound. By employing bioheat equation to model the ultrasound heat deposition, we can recover putative neural responses to ultrasound. Conclusions: The developed method for canceling transient thermal fluorescence quenching could also find applications with optical stimulation techniques to monitor thermal effects and disentangle them from neural responses. This approach may help deepen our understanding of the mechanisms and macroscopic effects of ultrasound neuromodulation, further paving the way for tailoring the stimulation regimes toward specific applications.
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published
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Journal / series
Volume
11 (1)
Pages / Article No.
14413
Publisher
SPIE
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Software
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Subject
ultrasound neuromodulation; thermal effects; neuroimaging; calcium imaging; mouse brain
Organisational unit
09648 - Razansky, Daniel / Razansky, Daniel