Aptamer-modified biosensors to visualize neurotransmitter flux
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Date
2022-01-01
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Chemical biosensors with the capacity to continuously monitor various neurotransmitter dynamics can be powerful tools to understand complex signaling pathways in the brain. However, in vivo detection of neurochemicals is challenging for many reasons such as the rapid release and clearance of neurotransmitters in the extracellular space, or the low target analyte concentrations in a sea of interfering biomolecules. Biosensing platforms with adequate spatiotemporal resolution coupled to specific and selective receptors termed aptamers, demonstrate high potential to tackle such challenges. Herein, we review existing literature in this field. We first discuss nanoparticle-based systems, which have a simple in vitro implementation and easily interpretable results. We then examine methods employing near-infrared detection for deeper tissue imaging, hence easier translation to in vivo implementation. We conclude by reviewing live cell imaging of neurotransmitter release via aptamermodified platforms. For each of these sensors, we discuss the associated challenges for translation to real-time in vivo neurochemical imaging. Realization of in vivo biosensors for neurotransmitters will drive future development of early prevention strategies, treatments, and therapeutics for psychiatric and neurodegenerative diseases.
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Publication status
published
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Book title
Journal / series
Volume
365
Pages / Article No.
109386
Publisher
Elsevier
Event
Edition / version
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Software
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Date collected
Date created
Subject
DNA aptamers; Optical sensors; Gold nanoparticles; Quantum dots; Near-infrared sensors; Live cell imaging; Real time; Serotonin; Dopamine; Histamine; Adenosine; Norepinephrine; Acetylcholine
Organisational unit
09640 - Wetter Slack, Emma / Wetter Slack, Emma
Notes
Funding
881603 - Graphene Flagship Core Project 3 (EC)