Comprehensive quantitative analysis of central carbon and amino-acid metabolism in Saccharomyces cerevisiae under multiple conditions by targeted proteomics
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Date
2011-01
Publication Type
Journal Article
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yes
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Abstract
Decades of biochemical research have identified most of the enzymes that catalyze metabolic reactions in the yeast Saccharomyces cerevisiae. The adaptation of metabolism to changing nutritional conditions, in contrast, is much less well understood. As an important stepping stone toward such understanding, we exploit the power of proteomics assays based on selected reaction monitoring (SRM) mass spectrometry to quantify abundance changes of the 228 proteins that constitute the central carbon and amino‐acid metabolic network in the yeast Saccharomyces cerevisiae, at five different metabolic steady states. Overall, 90% of the targeted proteins, including families of isoenzymes, were consistently detected and quantified in each sample, generating a proteomic data set that represents a nutritionally perturbed biological system at high reproducibility. The data set is near comprehensive because we detect 95–99% of all proteins that are required under a given condition. Interpreted through flux balance modeling, the data indicate that S. cerevisiae retains proteins not necessarily used in a particular environment. Further, the data suggest differential functionality for several metabolic isoenzymes.
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published
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Journal / series
Volume
7 (1)
Pages / Article No.
464
Publisher
Nature
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Software
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Subject
metabolism; S. cerevisiae; SRM; targeted proteomics
Organisational unit
03927 - Picotti, Paola / Picotti, Paola
03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
03713 - Sauer, Uwe / Sauer, Uwe