Phylogenetically closely related pseudomonads isolated from arthropods exhibit differential insect-killing abilities and genetic variations in insecticidal factors
OPEN ACCESS
Loading...
Author / Producer
Date
2021-09
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
OPEN ACCESS
Data
Abstract
Strains belonging to the Pseudomonas protegens and Pseudomonas chlororaphis species are able to control soilborne plant pathogens and to kill pest insects by producing virulence factors such as toxins, chitinases, antimicrobials or two-partner secretion systems. Most insecticidal Pseudomonas described so far were isolated from roots or soil. It is unknown whether these bacteria naturally occur in arthropods and how they interact with them. Therefore, we isolated P. protegens and P. chlororaphis from various healthy insects and myriapods, roots and soil collected in an agricultural field and a neighbouring grassland. The isolates were compared for insect killing, pathogen suppression and host colonization abilities. Our results indicate that neither the origin of isolation nor the phylogenetic position mirror the degree of insecticidal activity. Pseudomonas protegens strains appeared homogeneous regarding phylogeny, biocontrol and insecticidal capabilities, whereas P. chlororaphis strains were phylogenetically and phenotypically more heterogenous. A phenotypic and genomic analysis of five closely related P. chlororaphis isolates displaying varying levels of insecticidal activity revealed variations in genes encoding insecticidal factors that may account for the reduced insecticidal activity of certain isolates. Our findings point towards an adaption to insects within closely related pseudomonads and contribute to understand the ecology of insecticidal Pseudomonas.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
23 (9)
Pages / Article No.
5378 - 5394
Publisher
Wiley
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Organisational unit
03516 - McDonald, Bruce (emeritus) / McDonald, Bruce (emeritus)
Notes
Funding
184666 - Towards building microbial consortia for pest control: exploring adaptation to insect hosts and intraspecific diversity of pathogenicity and competition traits in insecticidal pseudomonads (SNF)
159520 - Molecular determinats contributing to insect and plant associated lifestyles of beneficial pseudomonads (SNF)
159520 - Molecular determinats contributing to insect and plant associated lifestyles of beneficial pseudomonads (SNF)
Related publications and datasets
Is supplemented by: https://doi.org/10.3929/ethz-b-000672650