The Gfr Uptake System Provides a Context-Dependent Fitness Advantage to Salmonella Typhimurium SL1344 During the Initial Gut Colonization Phase
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Date
2025-12
Publication Type
Journal Article
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yes
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Abstract
Salmonella enterica serovar Typhimurium (S. Tm) is a major cause of foodborne diarrhea. However, in healthy individuals, the microbiota typically restricts the growth of incoming pathogens, a protective mechanism termed colonization resistance (CR). To circumvent CR, Salmonella strains can utilize private nutrients that remain untapped by the resident microbiota. However, the metabolic pathways and environmental niches promoting pathogen growth are still not completely understood. Here, we investigate the significance of the gfr operon in gut colonization of S. Tm, which is essential for the utilization of fructoselysine (FL) and glucoselysine (GL). These Amadori compounds are present in heated foods with high protein and carbohydrate contents. We detected FL in both mouse chow and the intestinal tract of mice and showed that gfr mutants are attenuated during the initial phase of colonization in the murine model. Experiments in gnotobiotic mice and competition experiments with Escherichia coli suggest that gfr-dependent fitness advantage is context-dependent. We conclude that dietary Amadori products like FL can support S. Tm gut colonization, depending on the metabolic capacities of the microbiota.
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published
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Journal / series
Volume
124 (6)
Pages / Article No.
507 - 520
Publisher
Wiley
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Edition / version
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Software
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Date collected
Date created
Subject
Amadori products; Colonization resistance; Diet; Fructoselysine/glucoselysine; Microbial competition
Organisational unit
09583 - Sunagawa, Shinichi / Sunagawa, Shinichi
03980 - Piel, Jörn / Piel, Jörn
03740 - Vorholt, Julia / Vorholt, Julia
03589 - Hardt, Wolf-Dietrich / Hardt, Wolf-Dietrich
02520 - Institut für Mikrobiologie / Institute of Microbiology
Notes
Funding
192567 - Mechanisms controlling the Salmonella Typhimurium gut infection (SNF)
-180575 - NCCR Microbiomes SNF (SNF)
-225148 - NCCR Microbiomes in phase II (SNF)
-180575 - NCCR Microbiomes SNF (SNF)
-225148 - NCCR Microbiomes in phase II (SNF)