Simultaneous impedance spectroscopy and stimulation of human IPS-derived cardiac 3D spheroids in hanging-drop networks


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Date

2015

Publication Type

Conference Paper

ETH Bibliography

yes

Citations

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Data

Abstract

Here, we present electrical impedance spectroscopy (EIS) data of human iPS-derived cardiac 3D spheroids with electric stimulation integrated in a hanging drop network. Microscopy videos of the beating spheroids were correlated with synchronously obtained EIS recordings. For stimulation, the spheroid was exposed to a continuous sinusoidal electric field - in contrast to traditional pulse trains. This stimulating field was supplied via the same electrodes that were used for the EIS recordings. Our measurements revealed a beating frequency modulation upon tuning the stimulation signal amplitude.

Publication status

published

Editor

Book title

2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

Journal / series

Volume

Pages / Article No.

226 - 229

Publisher

IEEE

Event

28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

Edition / version

Methods

Software

Geographic location

Date collected

Date created

Subject

Organisational unit

03684 - Hierlemann, Andreas / Hierlemann, Andreas check_circle

Notes

Funding

296257 - The Body-on-a-Chip (BoC) (EC)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)

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