Determination of metabolic flux ratios from 13C-experiments and gas chromatography-mass spectrometry data
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Author / Producer
Date
2007
Publication Type
Book Chapter
ETH Bibliography
yes
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Abstract
Network topology is a necessary fundament to understand function and properties of microbial reaction networks. A valuable method for experimental elucidation of metabolic network topology is metabolic flux ratio analysis, which quantifies the relative contribution of two or more converging pathways to a given metabolite. It is based on 13C-labeling experiments, gas chromatography-mass spectrometry analysis, and probabilistic equations that relate mass distributions in proteinogenic amino acids to pathway activity. Here, we describe the protocol for sample generation and illustrate the principles underlying the calculation of metabolic flux ratios with three examples. These principles are also implemented in the publicly available software FiatFlux, which directly calculates flux ratios from the mass spectra of amino acids.
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Publication status
published
Book title
Metabolomics
Journal / series
Volume
358
Pages / Article No.
177 - 197
Publisher
Humana Press
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
METAFoR; metabolic flux analysis; carbon flux; central metabolism; mass isotopes
Organisational unit
03713 - Sauer, Uwe / Sauer, Uwe
03710 - Hafen, Ernst (emeritus) / Hafen, Ernst (emeritus)