Rethinking temperature effects on leaf growth, gene expression and metabolism: Diel variation matters
OPEN ACCESS
Loading...
Author / Producer
Date
2021-07
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
OPEN ACCESS
Data
Abstract
Plants have evolved to grow under prominently fluctuating environmental conditions. In experiments under controlled conditions, temperature is often set to artificial, binary regimes with constant values at day and at night. This study investigated how such a diel (24 hr) temperature regime affects leaf growth, carbohydrate metabolism and gene expression, compared to a temperature regime with a field‐like gradual increase and decline throughout 24 hr. Soybean (Glycine max) was grown under two contrasting diel temperature treatments. Leaf growth was measured in high temporal resolution. Periodical measurements were performed of carbohydrate concentrations, carbon isotopes as well as the transcriptome by RNA sequencing. Leaf growth activity peaked at different times under the two treatments, which cannot be explained intuitively. Under field‐like temperature conditions, leaf growth followed temperature and peaked in the afternoon, whereas in the binary temperature regime, growth increased at night and decreased during daytime. Differential gene expression data suggest that a synchronization of cell division activity seems to be evoked in the binary temperature regime. Overall, the results show that the coordination of a wide range of metabolic processes is markedly affected by the diel variation of temperature, which emphasizes the importance of realistic environmental settings in controlled condition experiments.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
44 (7)
Pages / Article No.
2262 - 2276
Publisher
Wiley
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
circadian; controlled conditions; fluctuating temperature; natural environment; storage carbohydrates
Organisational unit
03648 - Buchmann, Nina / Buchmann, Nina
03969 - Studer, Bruno / Studer, Bruno
03894 - Walter, Achim / Walter, Achim
Notes
Funding
153545 - Carbon isotope fractionation during leaf and root respiration as a function of nitrogen nutrition (SNF)
169542 - PhenoCOOL: Wheat and soybean phenotyping under cold conditions using growth as a dynamic trait (SNF)
169542 - PhenoCOOL: Wheat and soybean phenotyping under cold conditions using growth as a dynamic trait (SNF)