Investigation of Calcium Mechanotransduction by Quasi 3-D Microfiber Mechanical Stimulation of Cells
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Date
2008
Publication Type
Conference Paper
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no
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Abstract
Fibroblast cells mechanically interact with their environment through several mechanisms, including the formation of focal adhesion complexes (FACs) with adjacent ligands. A quasi-3D (Q3D) experimental device was devised to investigate chemo-mechanical stimulation of these cells. It employed a novel microscopic fixture featuring micropolymer arrays positioned just above a cellular monolayer, enabling mechanical stimulation through dorsal attachment to FACs. This protocol demonstrated the ability to preserve the normal stellate morphology of fibroblasts. Live-cell imaging during mechanical stimulation revealed robust calcium elevation in cells interacting with the Q3D device. This system provides a simple alternative to existing 3D culture methods yet allows dynamic, real-time mechanical probing of cells in a physiologically significant environment as compared to optical or magnetic tweezers.
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published
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Book title
Proceedings of the ASME 2008 Summer Bioengineering Conference, Parts A and B
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Pages / Article No.
1049 - 1050
Publisher
American Society of Mechanical Engineers
Event
ASME 2008 Summer Bioengineering Conference (SBC 2008)
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Organisational unit
09726 - Sitti, Metin (ehemalig) / Sitti, Metin (former)
Notes
Paper No: SBC2008-192913