Pervasive compartment-specific regulation of gene expression during homeostatic synaptic scaling
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Date
2021-10-05
Publication Type
Journal Article
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Abstract
Synaptic scaling is a form of homeostatic plasticity which allows neurons to adjust their action potential firing rate in response to chronic alterations in neural activity. Synaptic scaling requires profound changes in gene expression, but the relative contribution of local and cell-wide mechanisms is controversial. Here we perform a comprehensive multi-omics characterization of the somatic and process compartments of primary rat hippocampal neurons during synaptic scaling. We uncover both highly compartment-specific and correlating changes in the neuronal transcriptome and proteome. Whereas downregulation of crucial regulators of neuronal excitability occurs primarily in the somatic compartment, structural components of excitatory postsynapses are mostly downregulated in processes. Local inhibition of protein synthesis in processes during scaling is confirmed for candidate synaptic proteins. Motif analysis further suggests an important role for trans-acting post-transcriptional regulators, including RNA-binding proteins and microRNAs, in the local regulation of the corresponding mRNAs. Altogether, our study indicates that, during synaptic scaling, compartmentalized gene expression changes might co-exist with neuron-wide mechanisms to allow synaptic computation and homeostasis.
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published
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Volume
22 (10)
Pages / Article No.
Publisher
Wiley
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Subject
Cellular compartment; homeostatic plasticity; local translation; microRNA; synaptic scaling
Organisational unit
09499 - Bohacek, Johannes / Bohacek, Johannes
09498 - Schratt, Gerhard / Schratt, Gerhard
02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology
Notes
Funding
172889 - Dissecting stress-induced molecular changes in circuits underlying anxiety (SNF)
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