Classification of mouse B cell types using surfaceome proteotype maps
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Author / Producer
Date
2019
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.
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Publication status
published
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Editor
Book title
Journal / series
Volume
10 (1)
Pages / Article No.
5734
Publisher
Nature
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Organisational unit
02072 - Proteomics Plattform D-HEST
Notes
Funding
160259 - Direct identification of lateral protein interactions in the plasma membrane of living cells and tissues (SNF)