Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context
OPEN ACCESS
Author / Producer
Date
2020-08-07
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
OPEN ACCESS
Data
Rights / License
Abstract
The understanding of living systems and their building blocks relies heavily on the assessment of structure–function relationships at the nanoscale. Ever since the development of the first optical microscope, the reliance of scientists across disciplines on microscopy has increased. The development of the first electron microscope and with it the access to information at the nanoscale has prompted numerous disruptive discoveries. While fluorescence imaging allows identification of specific entities based on the labelling with fluorophores, the unlabelled constituents of the samples remain invisible. In electron microscopy on the other hand, structures can be comprehensively visualized based on their distinct electron density and geometry. Although electron microscopy is a powerful tool, it does not implicitly provide information on the location and activity of specific organic molecules. While correlative light and electron microscopy techniques have attempted to unify the two modalities, the resolution mismatch between the two data sets poses major challenges. Recent developments in optical super resolution microscopy enable high resolution correlative light and electron microscopy, however, with considerable constraints due to sample preparation requirements. Labelling of specific structures directly for electron microscopy using small gold nanoparticles (i.e. immunogold) has been used extensively. However, identification of specific entities solely based on electron contrast, and the differentiation from endogenous dense granules, remains challenging. Recently, the use of correlative cathodoluminescence electron microscopy (CCLEM) imaging based on luminescent inorganic nanocrystals has been proposed. While nanometric resolution can be reached for both the electron and the optical signal, high energy electron beams are potentially damaging to the sample. In this review, we discuss the opportunities of (volumetric) multi-color single protein labelling based on correlative cathodoluminescence electron microscopy, and its prospective impact on biomedical research in general. We elaborate on the potential challenges of correlative cathodoluminescence electron microscopy-based bioimaging and benchmark CCLEM against alternative high-resolution correlative imaging techniques.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
12 (29)
Pages / Article No.
15588 - 15603
Publisher
Royal Society of Chemistry
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Organisational unit
09675 - Herrmann, Inge Katrin (ehemalig) / Herrmann, Inge Katrin (former)
Notes
Funding
190832 - Multicolour Immunolabelling based on Cathodoluminescence Electron Microscopy (SNF)