jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths


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Date

2020-07

Publication Type

Journal Article

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yes

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Abstract

Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.

Publication status

published

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Volume

17 (7)

Pages / Article No.

694 - 697

Publisher

Nature

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