High-Speed Large-Field Multifocal Illumination Fluorescence Microscopy
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Date
2020-02
Publication Type
Journal Article
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yes
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Abstract
Scanning optical microscopy techniques are commonly restricted to a sub‐millimeter field‐of‐view (FOV) or otherwise employ slow mechanical translation, limiting their applicability for imaging fast biological dynamics occurring over large areas. A rapid scanning large‐field multifocal illumination (LMI) fluorescence microscopy technique is devised based on a beam‐splitting grating and an acousto‐optic deflector synchronized with a high‐speed camera to attain real‐time fluorescence microscopy over a centimeter‐scale FOV. Owing to its large depth of focus, the approach allows noninvasive visualization of perfusion across the entire mouse cerebral cortex, not achievable with conventional wide‐field fluorescence microscopy methods. The new concept can readily be incorporated into conventional wide‐field microscopes to mitigate image blur due to tissue scattering and attain optimal trade‐off between spatial resolution and FOV. It further establishes a bridge between conventional wide‐field macroscopy and laser scanning confocal microscopy, thus it is anticipated to find broad applicability in functional neuroimaging, in vivo cell tracking, and other applications looking at large‐scale fluorescent‐based biodynamics.
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Publication status
published
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Journal / series
Volume
14 (2)
Pages / Article No.
1900070
Publisher
Wiley
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Software
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Date collected
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Subject
Diffraction gratings; Fast scanning microscopy; Fluorescence imaging; Multifocal illumination
Organisational unit
09648 - Razansky, Daniel / Razansky, Daniel