A high yield cellulose extraction system for small whole wood samples and dual measurement of carbon and oxygen stable isotopes
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Date
2019-01-20
Publication Type
Journal Article
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yes
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Abstract
This paper describes devices to extract α-cellulose from small whole wood samples developed at the Lamont-Doherty Earth Observatory Tree-Ring Lab and explains the procedures for chemical extractions and for the dual analysis of carbon (δ13C) and oxygen (δ18O) stable isotopes. Here, we provide the necessary steps and guidelines for constructing a cellulose extraction system for small amounts of wood and leaves. The system allows the simultaneous extraction of cellulose from 150 samples by means of in-house filter tubes, where chemicals used for the cellulose extraction are exchanged and eliminated in batches. This new implementation diminishes the processing time, minimizes physical sample manipulation and potential errors, increases sample throughput, and reduces the amount of chemicals and analytic costs. We also describe the dual measurement of δ13C and δ18O ratios in tree-ring cellulose using high-temperature pyrolysis in a High Temperature Conversion Elemental Analyzer (TC/EA) interfaced with a Thermo Delta V plus mass spectrometer.
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published
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Book title
Journal / series
Volume
504
Pages / Article No.
53 - 65
Publisher
Elsevier
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Tree ring; α-Cellulose; Carbon stable isotopes (δ13C); Oxygen stable isotopes (δ18O); Continuous flow; High-temperature pyrolysis (HTP); Isotope ratio mass spectrometry (IRMS)
Organisational unit
08701 - Gruppe Waldbau / Group Silviculture
03535 - Bugmann, Harald / Bugmann, Harald
03535 - Bugmann, Harald / Bugmann, Harald