The Intestinal Microbiota Interferes with the microRNA Response upon Oral Listeria Infection
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Date
2013-12-10
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Journal Article
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Abstract
The intestinal tract is the largest reservoir of microbes in the human body. The intestinal microbiota is thought to be
able to modulate alterations of the gut induced by enteropathogens, thereby maintaining homeostasis. Listeria monocytogenes is
the agent of listeriosis, an infection transmitted to humans upon ingestion of contaminated food. Crossing of the intestinal barrier
is a critical step of the infection before dissemination into deeper organs. Here, we investigated the role of the intestinal microbiota
in the regulation of host protein-coding genes and microRNA (miRNA or miR) expression during Listeria infection. We
first established the intestinal miRNA signatures corresponding to the 10 most highly expressed miRNAs in the murine ileum of
conventional and germfree mice, noninfected and infected with Listeria. Next, we identified 6 miRNAs whose expression decreased
upon Listeria infection in conventional mice. Strikingly, five of these miRNA expression variations (in miR-143, miR-
148a, miR-200b, miR-200c, and miR-378) were dependent on the presence of the microbiota. In addition, as is already known,
protein-coding genes were highly affected by infection in both conventional and germfree mice. By crossing bioinformatically
the predicted targets of the miRNAs to our whole-genome transcriptomic data, we revealed an miRNA-mRNA network that suggested
miRNA-mediated global regulation during intestinal infection. Other recent studies have revealed an miRNA response to
either bacterial pathogens or commensal bacteria. In contrast, our work provides an unprecedented insight into the impact of
the intestinal microbiota on host transcriptional reprogramming during infection by a human pathogen.
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4 (6)
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American Society for Microbiology
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03983 - Ciaudo, C. (ehemalig) / Ciaudo, C. (former)