
Open access
Autor(in)
Barbier de Reuille, Pierre
Routier-Kierzkowska, Anne-Lise
Kierzkowski, Daniel
Bassel, George W.
Schüpbach, Thierry
Tauriello, Gerardo
Bajpai, Namrata
Strauss, Sören
Weber, Alain
Kiss, Annamaria
Burian, Agata
Hofhuis, Hugo
Sapala, Aleksandra
Lipowczan, Marcin
Heimlicher, Maria B.
Robinson, Sarah
Bayer, Emmanuelle M.
Basler, Konrad
Koumoutsakos, Petros
Roeder, Adrienne H.K.
Aegerter-Wilmsen, Tinri
Nakayama, Naomi
Tsiantis, Miltos
Hay, Angela
Kwiatkowska, Dorota
Xenarios, Ioannis
Kuhlemeier, Cris
Smith, Richard S.
Datum
2015-05Typ
- Journal Article
Abstract
Morphogenesis emerges from complex multiscale interactions between genetic and mechanical processes. To understand these processes, the evolution of cell shape, proliferation and gene expression must be quantified. This quantification is usually performed either in full 3D, which is computationally expensive and technically challenging, or on 2D planar projections, which introduces geometrical artifacts on highly curved organs. Here we present MorphoGraphX (www.MorphoGraphX.org), a software that bridges this gap by working directly with curved surface images extracted from 3D data. In addition to traditional 3D image analysis, we have developed algorithms to operate on curved surfaces, such as cell segmentation, lineage tracking and fluorescence signal quantification. The software's modular design makes it easy to include existing libraries, or to implement new algorithms. Cell geometries extracted with MorphoGraphX can be exported and used as templates for simulation models, providing a powerful platform to investigate the interactions between shape, genes and growth. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000101393Publikationsstatus
publishedZeitschrift / Serie
eLifeBand
Seiten / Artikelnummer
Verlag
eLife Sciences PublicationsOrganisationseinheit
03499 - Koumoutsakos, Petros / Koumoutsakos, Petros