Show simple item record

dc.contributor.author
Tyagi, Kshitiz
dc.contributor.author
Pedrioli, Patrick G.A.
dc.date.accessioned
2019-04-04T12:29:22Z
dc.date.available
2017-06-11T18:10:57Z
dc.date.available
2019-04-04T12:29:22Z
dc.date.issued
2015-05-19
dc.identifier.other
10.1093/nar/gkv322
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/102197
dc.identifier.doi
10.3929/ethz-b-000102197
dc.description.abstract
Maintenance of protein quality control has implications in various processes such as neurodegeneration and ageing. To investigate how environmental insults affect this process, we analysed the proteome of yeast continuously exposed to mild heat stress. In agreement with previous transcriptomics studies, amongst the most marked changes, we found up-regulation of cytoprotective factors; a shift from oxidative phosphorylation to fermentation; and down-regulation of translation. Importantly, we also identified a novel, post-translationally controlled, component of the heat shock response. The abundance of Ncs2p and Ncs6p, two members of the URM1 pathway responsible for the thiolation of wobble uridines in cytoplasmic tRNAs tKUUU, tQUUG and tEUUC, is down-regulated in a proteasomal dependent fashion. Using random forests we show that this results in differential translation of transcripts with a biased content for the corresponding codons. We propose that the role of this pathway in promoting catabolic and inhibiting anabolic processes, affords cells with additional time and resources needed to attain proper protein folding under periods of stress.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Protein degradation and dynamic tRNA thiolation fine-tune translation at elevated temperatures
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2015-04-13
ethz.journal.title
Nucleic acids research
ethz.journal.volume
43
en_US
ethz.journal.issue
9
en_US
ethz.pages.start
4701
en_US
ethz.pages.end
4712
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
000038633
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T18:11:47Z
ethz.source
ECIT
ethz.identifier.importid
imp5936534ac7bb738505
ethz.ecitpid
pub:160324
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T19:01:27Z
ethz.rosetta.lastUpdated
2019-04-04T12:29:48Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Protein%20degradation%20and%20dynamic%20tRNA%20thiolation%20fine-tune%20translation%20at%20elevated%20temperatures&rft.jtitle=Nucleic%20acids%20research&rft.date=2015-05-19&rft.volume=43&rft.issue=9&rft.spage=4701&rft.epage=4712&rft.au=Tyagi,%20Kshitiz&Pedrioli,%20Patrick%20G.A.&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record