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dc.contributor.author
Svozil, Julia
dc.contributor.author
Gruissem, Wilhelm
dc.contributor.author
Baerenfaller, Katja
dc.date.accessioned
2019-06-06T09:53:07Z
dc.date.available
2017-06-11T18:15:04Z
dc.date.available
2019-06-06T09:53:07Z
dc.date.issued
2015-05
dc.identifier.issn
1664-462X
dc.identifier.other
10.3389/fpls.2015.00376
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/102354
dc.identifier.doi
10.3929/ethz-b-000102354
dc.description.abstract
Protein and transcript levels are partly decoupled as a function of translation efficiency and protein degradation. Selective protein degradation via the Ubiquitin-26S proteasome system (UPS) ensures protein homeostasis and facilitates adjustment of protein abundance during changing environmental conditions. Since individual leaf tissues have specialized functions, their protein composition is different and hence also protein level regulation is expected to differ. To understand UPS function in a tissue-specific context we developed a method termed Meselect to effectively and rapidly separate Arabidopsis thaliana leaf epidermal, vascular and mesophyll tissues. Epidermal and vascular tissue cells are separated mechanically, while mesophyll cells are obtained after rapid protoplasting. The high yield of proteins was sufficient for tissue-specific proteome analyses after inhibition of the proteasome with the specific inhibitor Syringolin A (SylA) and affinity enrichment of ubiquitylated proteins. SylA treatment of leaves resulted in the accumulation of 225 proteins and identification of 519 ubiquitylated proteins. Proteins that were exclusively identified in the three different tissue types are consistent with specific cellular functions. Mesophyll cell proteins were enriched for plastid membrane translocation complexes as targets of the UPS. Epidermis enzymes of the TCA cycle and cell wall biosynthesis specifically accumulated after proteasome inhibition, and in the vascular tissue several enzymes involved in glucosinolate biosynthesis were found to be ubiquitylated. Our results demonstrate that protein level changes and UPS protein targets are characteristic of the individual leaf tissues and that the proteasome is relevant for tissue-specific functions.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
protein level regulation
en_US
dc.subject
ubiquitylation
en_US
dc.subject
proteasome inhibition
en_US
dc.subject
leaf tissues
en_US
dc.subject
epidermis
en_US
dc.subject
mesophyll
en_US
dc.subject
vasculature
en_US
dc.subject
Arabidopsis thaliana
en_US
dc.title
Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2015-05-28
ethz.journal.title
Frontiers in Plant Science
ethz.journal.volume
6
en_US
ethz.journal.abbreviated
Front. Plant Sci.
ethz.pages.start
376
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
010181292
ethz.publication.place
Lausanne
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03554 - Gruissem, Wilhelm (emeritus) / Gruissem, Wilhelm (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03554 - Gruissem, Wilhelm (emeritus) / Gruissem, Wilhelm (emeritus)
ethz.date.deposited
2017-06-11T18:15:23Z
ethz.source
ECIT
ethz.identifier.importid
imp5936534e5410374994
ethz.ecitpid
pub:160486
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T08:08:13Z
ethz.rosetta.lastUpdated
2024-02-02T08:13:32Z
ethz.rosetta.versionExported
true
ethz.COinS
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