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dc.contributor.author
Monfort, Asun
dc.contributor.author
Di Minin, Giulio
dc.contributor.author
Postlmayr, Andreas
dc.contributor.author
Freimann, Remo
dc.contributor.author
Arieti, Fabiana
dc.contributor.author
Thore, Stéphane
dc.contributor.author
Wutz, Anton
dc.date.accessioned
2018-09-19T14:19:34Z
dc.date.available
2017-06-11T18:33:07Z
dc.date.available
2018-09-19T14:19:34Z
dc.date.issued
2015-07
dc.identifier.issn
2666-3864
dc.identifier.issn
2211-1247
dc.identifier.other
10.1016/j.celrep.2015.06.067
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/102870
dc.identifier.doi
10.3929/ethz-b-000102870
dc.description.abstract
In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X chromosomes in female cells. Here, we report a genetic screen for silencing factors in X chromosome inactivation using haploid mouse embryonic stem cells (ESCs) that carry an engineered selectable reporter system. This system was able to identify several candidate factors that are genetically required for chromosomal repression by Xist. Among the list of candidates, we identify the RNA-binding protein Spen, the homolog of split ends. Independent validation through gene deletion in ESCs confirms that Spen is required for gene repression by Xist. However, Spen is not required for Xist RNA localization and the recruitment of chromatin modifications, including Polycomb protein Ezh2. The identification of Spen opens avenues for further investigation into the gene-silencing pathway of Xist and shows the usefulness of haploid ESCs for genetic screening of epigenetic pathways.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title
Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2015-07-16
ethz.journal.title
Cell Reports
ethz.journal.volume
12
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Cell Rep
ethz.pages.start
554
en_US
ethz.pages.end
561
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
X chromosome reactivation in development and transformation
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007612702
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
ethz.grant.agreementno
152814
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2017-06-11T18:33:11Z
ethz.source
ECIT
ethz.identifier.importid
imp5936535a7ee1849561
ethz.ecitpid
pub:161030
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T12:00:52Z
ethz.rosetta.lastUpdated
2022-03-28T21:20:36Z
ethz.rosetta.versionExported
true
ethz.COinS
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