Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix

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Date
2015Type
- Journal Article
Citations
Cited 124 times in
Web of Science
Cited 128 times in
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ETH Bibliography
yes
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Abstract
Despite the crucial role of extracellular matrix (ECM) in directing cell fate in healthy and diseased tissues—particularly in development, wound healing, tissue regeneration and cancer—the mechanisms that direct the assembly and regulate hierarchical architectures of ECM are poorly understood. Collagen I matrix assembly in vivo requires active fibronectin (Fn) fibrillogenesis by cells. Here we exploit Fn-FRET probes as mechanical strain sensors and demonstrate that collagen I fibres preferentially co-localize with more-relaxed Fn fibrils in the ECM of fibroblasts in cell culture. Fibre stretch-assay studies reveal that collagen I’s Fn-binding domain is responsible for the mechano-regulated interaction. Furthermore, we show that Fn-collagen interactions are reciprocal: relaxed Fn fibrils act as multivalent templates for collagen assembly, but once assembled, collagen fibres shield Fn fibres from being stretched by cellular traction forces. Thus, in addition to the well-recognized, force-regulated, cell-matrix interactions, forces also tune the interactions between different structural ECM components. Show more
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https://doi.org/10.3929/ethz-b-000103188Publication status
publishedJournal / series
Nature CommunicationsVolume
Pages / Article No.
Publisher
Nature Publishing GroupOrganisational unit
03640 - Vogel, Viola / Vogel, Viola
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Show all metadata
Citations
Cited 124 times in
Web of Science
Cited 128 times in
Scopus
ETH Bibliography
yes
Altmetrics