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dc.contributor.author
Kubow, Kristopher E.
dc.contributor.author
Vukmirovic, Radmila
dc.contributor.author
Lin, Zhe
dc.contributor.author
Klotzsch, Enrico
dc.contributor.author
Smith, Michael L.
dc.contributor.author
Gourdon, Delphine
dc.contributor.author
Luna, Sheila
dc.contributor.author
Vogel, Viola
dc.date.accessioned
2018-09-12T11:44:20Z
dc.date.available
2017-06-11T18:45:17Z
dc.date.available
2018-09-12T11:44:20Z
dc.date.issued
2015
dc.identifier.other
10.1038/ncomms9026
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/103188
dc.identifier.doi
10.3929/ethz-b-000103188
dc.description.abstract
Despite the crucial role of extracellular matrix (ECM) in directing cell fate in healthy and diseased tissues—particularly in development, wound healing, tissue regeneration and cancer—the mechanisms that direct the assembly and regulate hierarchical architectures of ECM are poorly understood. Collagen I matrix assembly in vivo requires active fibronectin (Fn) fibrillogenesis by cells. Here we exploit Fn-FRET probes as mechanical strain sensors and demonstrate that collagen I fibres preferentially co-localize with more-relaxed Fn fibrils in the ECM of fibroblasts in cell culture. Fibre stretch-assay studies reveal that collagen I’s Fn-binding domain is responsible for the mechano-regulated interaction. Furthermore, we show that Fn-collagen interactions are reciprocal: relaxed Fn fibrils act as multivalent templates for collagen assembly, but once assembled, collagen fibres shield Fn fibres from being stretched by cellular traction forces. Thus, in addition to the well-recognized, force-regulated, cell-matrix interactions, forces also tune the interactions between different structural ECM components.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2015-08-14
ethz.journal.title
Nature Communications
ethz.journal.volume
6
en_US
ethz.pages.start
8026
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007044158
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.date.deposited
2017-06-11T18:46:08Z
ethz.source
ECIT
ethz.identifier.importid
imp593653620d66722889
ethz.ecitpid
pub:161389
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T19:57:15Z
ethz.rosetta.lastUpdated
2019-02-03T07:54:20Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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