- Book Chapter
Membrane proteins are an important class of proteins in biology and therapeutics. Understanding the dynamic nature of the molecular interactions that stabilize membrane protein structure is critical to dissect the mechanism of action and dysfunction of these proteins. Single-molecule force spectroscopy (SMFS) and dynamic SMFS (DFS) are emerging nanotechniques that allow the study of membrane proteins under the physiologically relevant conditions of a lipid bilayer and buffer conditions. These techniques directly probe the molecular interactions underlying protein structure and reveal unique insights about their properties. Outlined in this report will be procedures on how to conduct SMFS and DFS on rhodopsin in native retinal membranes. Rhodopsin is a membrane protein belonging to the G protein-coupled receptor family of proteins, one of the largest families of proteins in nature. Show more
Journal / seriesMethods in Molecular Biology
Pages / Article No.
SubjectAtomic force microscopy; Biological membranes; Energy landscape; Membrane proteins; Intramolecular interactions; Protein stability; Protein unfolding
Organisational unit03870 - Müller, Daniel J. / Müller, Daniel J.
MoreShow all metadata