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dc.contributor.author
Gasic, Ivana
dc.contributor.author
Nerurkar, Purnima
dc.contributor.author
Meraldi, Patrick
dc.date.accessioned
2018-09-10T12:28:11Z
dc.date.available
2017-06-11T20:01:00Z
dc.date.available
2018-09-10T12:28:11Z
dc.date.issued
2015-08
dc.identifier.issn
2050-084X
dc.identifier.other
10.7554/eLife.07909
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/105389
dc.identifier.doi
10.3929/ethz-b-000105389
dc.description.abstract
The poles of the mitotic spindle contain one old and one young centrosome. In asymmetric stem cell divisions, the age of centrosomes affects their behaviour and their probability to remain in the stem cell. In contrast, in symmetric divisions, old and young centrosomes are thought to behave equally. This hypothesis is, however, untested. In this study, we show in symmetrically dividing human cells that kinetochore–microtubules associated to old centrosomes are more stable than those associated to young centrosomes, and that this difference favours the accumulation of premature end-on attachments that delay the alignment of polar chromosomes at old centrosomes. This differential microtubule stability depends on cenexin, a protein enriched on old centrosomes. It persists throughout mitosis, biasing chromosome segregation in anaphase by causing daughter cells with old centrosomes to retain non-disjoint chromosomes 85% of the time. We conclude that centrosome age imposes via cenexin a functional asymmetry on all mitotic spindles.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
eLife Sciences Publications
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Centrosome age regulates kinetochore–microtubule stability and biases chromosome mis-segregation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
eLife
ethz.journal.volume
4
en_US
ethz.pages.start
e07909
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Investigating the global activities of kinetochores during human cell division
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007613147
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.grant.agreementno
141256
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2017-06-11T20:01:40Z
ethz.source
ECIT
ethz.identifier.importid
imp5936539188e6477213
ethz.ecitpid
pub:165003
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T23:56:18Z
ethz.rosetta.lastUpdated
2022-03-28T21:13:03Z
ethz.rosetta.versionExported
true
ethz.COinS
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