Computer-assisted quantification of motile and invasive capabilities of cancer cells
Kumar, Karthiga S.
Grotzer, Michael A.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
High-throughput analysis of cancer cell dissemination and its control by extrinsic and intrinsic cellular factors is hampered by the lack of adequate and efficient analytical tools for quantifying cell motility. Oncology research would greatly benefit from such a methodology that allows to rapidly determine the motile behaviour of cancer cells under different environmental conditions, including inside three-dimensional matrices. We combined automated microscopy imaging of two- and three-dimensional cell cultures with computational image analysis into a single assay platform for studying cell dissemination in high-throughput. We have validated this new approach for medulloblastoma, a metastatic paediatric brain tumour, in combination with the activation of growth factor signalling pathways with established pro-migratory functions. The platform enabled the detection of primary tumour and patient-derived xenograft cell sensitivity to growth factor-dependent motility and dissemination and identified tumour subgroup-specific responses to selected growth factors of excellent diagnostic value. Show more
Journal / seriesScientific Reports
Pages / Article No.
PublisherNature Publishing Group
SubjectPaediatric cancer; Experimental models of disease; Embryonal neoplasms; Cell invasion
Organisational unit03852 - Schneider, Gisbert / Schneider, Gisbert
134783 - Computergestützer Entwurf und biophysikalische Charakterisierung immunmodulatorischer und antibakterieller Peptide und Peptidomimetika (SNF)
144091 - Understanding and exploiting the molecular targeting of tumor neo-vasculature (SNF)
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