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dc.contributor.author
Kumar, Karthiga S.
dc.contributor.author
Pillong, Max
dc.contributor.author
Kunze, Jens
dc.contributor.author
Burghardt, Isabel
dc.contributor.author
Weller, Michael
dc.contributor.author
Grotzer, Michael A.
dc.contributor.author
Schneider, Gisbert
dc.contributor.author
Baumgartner, Martin
dc.date.accessioned
2018-10-16T12:01:46Z
dc.date.available
2017-06-11T20:24:13Z
dc.date.available
2018-10-16T12:01:46Z
dc.date.issued
2015-10-21
dc.identifier.other
10.1038/srep15338
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/106000
dc.identifier.doi
10.3929/ethz-b-000106000
dc.description.abstract
High-throughput analysis of cancer cell dissemination and its control by extrinsic and intrinsic cellular factors is hampered by the lack of adequate and efficient analytical tools for quantifying cell motility. Oncology research would greatly benefit from such a methodology that allows to rapidly determine the motile behaviour of cancer cells under different environmental conditions, including inside three-dimensional matrices. We combined automated microscopy imaging of two- and three-dimensional cell cultures with computational image analysis into a single assay platform for studying cell dissemination in high-throughput. We have validated this new approach for medulloblastoma, a metastatic paediatric brain tumour, in combination with the activation of growth factor signalling pathways with established pro-migratory functions. The platform enabled the detection of primary tumour and patient-derived xenograft cell sensitivity to growth factor-dependent motility and dissemination and identified tumour subgroup-specific responses to selected growth factors of excellent diagnostic value.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Paediatric cancer
en_US
dc.subject
Experimental models of disease
en_US
dc.subject
Embryonal neoplasms
en_US
dc.subject
Cell invasion
en_US
dc.title
Computer-assisted quantification of motile and invasive capabilities of cancer cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
5
en_US
ethz.pages.start
15338
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Computergestützer Entwurf und biophysikalische Charakterisierung immunmodulatorischer und antibakterieller Peptide und Peptidomimetika
en_US
ethz.grant
Understanding and exploiting the molecular targeting of tumor neo-vasculature
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03852 - Schneider, Gisbert / Schneider, Gisbert
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03852 - Schneider, Gisbert / Schneider, Gisbert
ethz.grant.agreementno
134783
ethz.grant.agreementno
144091
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Mathematik, Natur- und Ingenieurwissenschaften (Abteilung II)
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2017-06-11T20:24:43Z
ethz.source
ECIT
ethz.identifier.importid
imp5936539dd9d4351931
ethz.ecitpid
pub:165948
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T20:11:57Z
ethz.rosetta.lastUpdated
2019-01-02T14:34:31Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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