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dc.contributor.author
Vasudevan, Kumar
dc.contributor.author
Gruissem, Wilhelm
dc.contributor.author
Bhullar, Navreet K.
dc.date.accessioned
2018-10-12T16:05:05Z
dc.date.available
2017-06-11T20:30:50Z
dc.date.available
2018-10-12T16:05:05Z
dc.date.issued
2015-10-26
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/srep15678
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/106237
dc.identifier.doi
10.3929/ethz-b-000106237
dc.description.abstract
Rice blast is one of the most devastating rice diseases and continuous resistance breeding is required to control the disease. The rice blast resistance gene Pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India. We explored the allelic diversity of the Pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M. oryzae strains as well as against five unique strains. These genotypes are also annotated as rice blast resistant in the International Rice Genebank database. Sequence-based allele mining was used to amplify and clone the Pi54 allelic variants. Nine new alleles of Pi54 were identified based on the nucleotide sequence comparison to the Pi54 reference sequence as well as to already known Pi54 alleles. DNA sequence analysis of the newly identified Pi54 alleles revealed several single polymorphic sites, three double deletions and an eight base pair deletion. A SNP-rich region was found between a tyrosine kinase phosphorylation site and the nucleotide binding site (NBS) domain. Together, the newly identified Pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Plant molecular biology
en_US
dc.subject
Plant immunity
en_US
dc.title
Identification of novel alleles of the rice blast resistance gene Pi54
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
5
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
15678
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03554 - Gruissem, Wilhelm (emeritus) / Gruissem, Wilhelm (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03554 - Gruissem, Wilhelm (emeritus) / Gruissem, Wilhelm (emeritus)
ethz.date.deposited
2017-06-11T20:30:53Z
ethz.source
ECIT
ethz.identifier.importid
imp593653a300e2046646
ethz.ecitpid
pub:166343
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-17T11:12:07Z
ethz.rosetta.lastUpdated
2024-02-02T06:21:00Z
ethz.rosetta.versionExported
true
ethz.COinS
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