Show simple item record

dc.contributor.author
Waschkies, Conny
dc.contributor.author
Nicholls, Flora
dc.contributor.author
Buschmann, Johanna
dc.date.accessioned
2018-10-16T12:30:02Z
dc.date.available
2017-06-11T20:32:59Z
dc.date.available
2018-10-16T12:30:02Z
dc.date.issued
2015-10-23
dc.identifier.other
10.1038/srep15536
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/106252
dc.identifier.doi
10.3929/ethz-b-000106252
dc.description.abstract
Non-invasive assessment of the perfusion capacity of tissue engineered constructs grown on the chorioallantoic membrane by MRI is often hampered by motion artifacts. Therefore, we examined the suitability of three anesthetic regimes for sufficient sedation of the chick embryo. Medetomidine at a dosage of 0.3 mg/kg, was compared to thiopental at 100 mg/kg and ketamine/midazolam at 50 mg/kg and 1 mg/kg, respectively. These soluble anesthetics were applied by dropping a total volume of 0.3 mL onto the surface of the CAM. Motion was videotaped through the window of the eggshell and scored semi-quantitatively. Medetomidine performed best in terms of reduced motion; onset of anesthesia occurred within 10 minutes and for the following 30 minutes, allowing proper in vivo MRI measurements. The other regimen were not sedating deep enough (ketamine/midazolam) and not long enough (thiopental). In sum, medetomidine allows proper sedation for MRI assessment of the perfusion capacity in a tissue engineered construct placed on the CAM.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Translational research
en_US
dc.subject
Tendons
en_US
dc.title
Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
5
en_US
ethz.pages.start
15536
en_US
ethz.size
6 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T20:33:57Z
ethz.source
ECIT
ethz.identifier.importid
imp593653a347b2372368
ethz.ecitpid
pub:166358
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T14:42:18Z
ethz.rosetta.lastUpdated
2019-01-02T14:34:50Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Comparison%20of%20medetomidine,%20thiopental%20and%20ketamine/midazolam%20anesthesia%20in%20chick%20embryos%20for%20in%20ovo%20Magnetic%20Resonance%20Imaging%20free%20of&rft.jtitle=Scientific%20Reports&rft.date=2015-10-23&rft.volume=5&rft.spage=15536&rft.au=Waschkies,%20Conny&Nicholls,%20Flora&Buschmann,%20Johanna&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record