Reassessment of MxiH subunit orientation and fold within native Shigella T3SS needles using surface labelling and solid-state NMR
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
T3SSs are essential virulence determinants of many Gram-negative bacteria, used to inject bacterial effectors of virulence into eukaryotic host cells. Their major extracellular portion, a ∼50 nm hollow, needle-like structure, is essential to host cell sensing and the conduit for effector secretion. It is formed of a small, conserved subunit arranged as a helical polymer. The structure of the subunit has been studied by electron cryomicroscopy within native polymers and by solid-state NMR in recombinant polymers, yielding two incompatible atomic models. To resolve this controversy, we re-examined the native polymer used for electron cryomicroscopy via surface labelling and solid-state NMR. Our data show the orientation and overall fold of the subunit within this polymer is as established by solid-state NMR for recombinant polymers. Show more
Journal / seriesJournal of Structural Biology
Pages / Article No.
SubjectNeedle; Shigella; Solid-state NMR; Surface labelling; Type III secretion
Organisational unit03496 - Meier, Beat H. / Meier, Beat H.
146757 - NMR studies in the Solid State (SNF)
159707 - NMR studies in the Solid State (SNF)
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