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dc.contributor.author
Maharjan, Ram P.
dc.contributor.author
Gaffé, Joël
dc.contributor.author
Plucain, Jessica
dc.contributor.author
Schliep, Martin
dc.contributor.author
Wang, Lei
dc.contributor.author
Feng, Lu
dc.contributor.author
Tenaillon, Olivier
dc.contributor.author
Ferenci, Thomas
dc.contributor.author
Schneider, Dominique
dc.date.accessioned
2018-10-09T10:00:12Z
dc.date.available
2017-06-11T21:50:49Z
dc.date.available
2018-10-09T10:00:12Z
dc.date.issued
2013-07
dc.identifier.other
10.1186/1471-2164-14-441
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/108237
dc.identifier.doi
10.3929/ethz-b-000108237
dc.description.abstract
Background DNA duplications constitute important precursors for genome variation. Here we analyzed an unequal duplication harboring a beneficial mutation that may provide alternative evolutionary outcomes. Results We characterized this evolutionary event during experimental evolution for only 100 generations of an Escherichia coli strain under glucose limitation within chemostats. By combining Insertion Sequence based Restriction Length Polymorphism experiments, pulsed field gel electrophoresis and two independent genome re-sequencing experiments, we identified an evolved lineage carrying a 180 kb duplication of the 46’ region of the E. coli chromosome. This evolved duplication revealed a heterozygous state, with one copy harboring a 2668 bp deletion that included part of the ogrK gene and both the yegR and yegS genes. By genetically manipulating ancestral and evolved strains, we showed that the single yegS inactivation was sufficient to confer a frequency dependent fitness increase under the chemostat selective conditions in both the ancestor and evolved genetic contexts, implying that the duplication itself was not a direct fitness contributor. Nonetheless, the heterozygous duplicated state was relatively stable in the conditions prevailing during evolution in chemostats, in striking contrast to non selective conditions in which the duplication resolved at high frequency into either its ancestral or deleted copy. Conclusions Our results suggest that the duplication state may constitute a second order selection process providing higher evolutionary potential. Moreover, its heterozygous nature may provide differential evolutionary opportunities in alternating environments. Our results also highlighted how careful analyses of whole genome data are needed to identify such complex rearrangements.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Experimental evolution
en_US
dc.subject
Chemostats
en_US
dc.subject
Escherichia coli
en_US
dc.subject
Duplication
en_US
dc.subject
Heterozygous duplication
en_US
dc.subject
Insertion sequence elements
en_US
dc.subject
Frequency-dependent selection
en_US
dc.subject
Indirect selection
en_US
dc.title
A case of adaptation through a mutation in a tandem duplication during experimental evolution in Escherichia coli
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
BMC Genomics
ethz.journal.volume
14
en_US
ethz.pages.start
441
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004256340
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03939 - Velicer, Gregory J. / Velicer, Gregory J.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03939 - Velicer, Gregory J. / Velicer, Gregory J.
ethz.date.deposited
2017-06-11T21:51:44Z
ethz.source
ECIT
ethz.identifier.importid
imp593653cab51b774024
ethz.ecitpid
pub:169034
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T18:28:12Z
ethz.rosetta.lastUpdated
2018-10-09T10:00:18Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
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