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dc.contributor.author
Zhu, Zhen
dc.contributor.author
Frey, Olivier
dc.contributor.author
Haandbaek, Niels
dc.contributor.author
Franke, Felix
dc.contributor.author
Rudolf, Fabian
dc.contributor.author
Hierlemann, Andreas
dc.date.accessioned
2018-09-18T15:35:52Z
dc.date.available
2017-06-11T21:54:55Z
dc.date.available
2018-09-18T15:35:52Z
dc.date.issued
2015-11-26
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/srep17180
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/108322
dc.identifier.doi
10.3929/ethz-b-000108322
dc.description.abstract
As a complement and alternative to optical methods, wide-band electrical impedance spectroscopy (EIS) enables multi-parameter, label-free and real-time detection of cellular and subcellular features. We report on a microfluidics-based system designed to reliably capture single rod-shaped Schizosaccharomyces pombe cells by applying suction through orifices in a channel wall. The system enables subsequent culturing of immobilized cells in an upright position, while dynamic changes in cell-cycle state and morphology were continuously monitored through EIS over a broad frequency range. Besides measuring cell growth, clear impedance signals for nuclear division have been obtained. The EIS system has been characterized with respect to sensitivity and detection limits. The spatial resolution in measuring cell length was 0.25 μm, which corresponds to approximately a 5-min interval of cell growth under standard conditions. The comprehensive impedance data sets were also used to determine the occurrence of nuclear division and cytokinesis. The obtained results have been validated through concurrent confocal imaging and plausibilized through comparison with finite-element modeling data. The possibility to monitor cellular and intracellular features of single S. pombe cells during the cell cycle at high spatiotemporal resolution renders the presented microfluidics-based EIS system a suitable tool for dynamic single-cell investigations.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Lab-on-a-chip
en_US
dc.subject
Biomedical engineering
en_US
dc.title
Time-lapse electrical impedance spectroscopy for monitoring the cell cycle of single immobilized S-pombe cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
5
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
17180
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Seamless Integration of Neurons with CMOS Microelectronics
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03699 - Stelling, Jörg / Stelling, Jörg
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03699 - Stelling, Jörg / Stelling, Jörg
ethz.grant.agreementno
267351
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2017-06-11T21:55:53Z
ethz.source
ECIT
ethz.identifier.importid
imp593653cc7568474378
ethz.ecitpid
pub:169275
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T10:54:52Z
ethz.rosetta.lastUpdated
2021-02-15T01:47:35Z
ethz.rosetta.versionExported
true
ethz.COinS
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