A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
Metadata only
Date
2015Type
- Book Chapter
ETH Bibliography
yes
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Abstract
Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA). Show more
Publication status
publishedEditor
Book title
Embryonic Stem Cell ProtocolsJournal / series
Methods in Molecular BiologyVolume
Pages / Article No.
Publisher
Humana PressEdition / version
Third editionSubject
Transfection; Human embryonic stem cells; Lipofection; siRNA mediated-knockdown; Gene silencingOrganisational unit
03978 - Wutz, Anton / Wutz, Anton
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ETH Bibliography
yes
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