Show simple item record

dc.contributor.author
Renz, Peter F.
dc.contributor.author
Beyer, Tobias Andreas
dc.contributor.editor
Turksen, Kursad
dc.date.accessioned
2021-07-12T14:03:57Z
dc.date.available
2017-06-11T23:02:15Z
dc.date.available
2021-07-12T14:03:57Z
dc.date.issued
2015
dc.identifier.isbn
978-1-4939-7976-9
en_US
dc.identifier.isbn
978-1-4939-2953-5
en_US
dc.identifier.isbn
978-1-4939-2954-2
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/7651_2015_212
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/110218
dc.description.abstract
Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).
en_US
dc.language.iso
en
en_US
dc.publisher
Humana Press
en_US
dc.subject
Transfection
en_US
dc.subject
Human embryonic stem cells
en_US
dc.subject
Lipofection
en_US
dc.subject
siRNA mediated-knockdown
en_US
dc.subject
Gene silencing
en_US
dc.title
A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
en_US
dc.type
Book Chapter
dc.date.published
2015-02-28
ethz.book.title
Embryonic Stem Cell Protocols
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
1341
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
369
en_US
ethz.pages.end
376
en_US
ethz.version.edition
Third edition
en_US
ethz.identifier.nebis
000916990
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
ethz.date.deposited
2017-06-11T23:02:54Z
ethz.source
ECIT
ethz.identifier.importid
imp593653efef75137521
ethz.ecitpid
pub:171404
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-18T17:43:10Z
ethz.rosetta.lastUpdated
2022-03-29T10:22:41Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=A%20Concise%20Protocol%20for%20siRNA-Mediated%20Gene%20Suppression%20in%20Human%20Embryonic%20Stem%20Cells&rft.jtitle=Methods%20in%20Molecular%20Biology&rft.date=2015&rft.volume=1341&rft.spage=369&rft.epage=376&rft.issn=1064-3745&1940-6029&rft.au=Renz,%20Peter%20F.&Beyer,%20Tobias%20Andreas&rft.isbn=978-1-4939-7976-9&978-1-4939-2953-5&978-1-4939-2954-2&rft.genre=bookitem&rft_id=info:doi/10.1007/7651_2015_212&rft.btitle=Embryonic%20Stem%20Cell%20Protocols
 Search print copy at ETH Library

Files in this item

FilesSizeFormatOpen in viewer

There are no files associated with this item.

Publication type

Show simple item record