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dc.contributor.author
Hehl, Adrian B.
dc.contributor.author
Basso, Walter U.
dc.contributor.author
Lippuner, Christoph
dc.contributor.author
Ramakrishnan, Chandra
dc.contributor.author
Okoniewski, Michal J.
dc.contributor.author
Walker, Robert A.
dc.contributor.author
Grigg, Michael E.
dc.contributor.author
Smith, Nicholas C.
dc.contributor.author
Deplazes, Peter
dc.date.accessioned
2018-10-08T16:12:39Z
dc.date.available
2017-06-11T23:19:12Z
dc.date.available
2018-10-08T16:12:39Z
dc.date.issued
2015-02
dc.identifier.other
10.1186/s12864-015-1225-x
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/110813
dc.identifier.doi
10.3929/ethz-b-000110813
dc.description.abstract
Background The apicomplexan parasite Toxoplasma gondii is cosmopolitan in nature, largely as a result of its highly flexible life cycle. Felids are its only definitive hosts and a wide range of mammals and birds serve as intermediate hosts. The latent bradyzoite stage is orally infectious in all warm-blooded vertebrates and establishes chronic, transmissible infections. When bradyzoites are ingested by felids, they transform into merozoites in enterocytes and expand asexually as part of their coccidian life cycle. In all other intermediate hosts, however, bradyzoites differentiate exclusively to tachyzoites, and disseminate extraintestinally to many cell types. Both merozoites and tachyzoites undergo rapid asexual population expansion, yet possess different effector fates with respect to the cells and tissues they develop in and the subsequent stages they differentiate into. Results To determine whether merozoites utilize distinct suites of genes to attach, invade, and replicate within feline enterocytes, we performed comparative transcriptional profiling on purified tachyzoites and merozoites. We used high-throughput RNA-Seq to compare the merozoite and tachyzoite transcriptomes. 8323 genes were annotated with sequence reads across the two asexually replicating stages of the parasite life cycle. Metabolism was similar between the two replicating stages. However, significant stage-specific expression differences were measured, with 312 transcripts exclusive to merozoites versus 453 exclusive to tachyzoites. Genes coding for 177 predicted secreted proteins and 64 membrane- associated proteins were annotated as merozoite-specific. The vast majority of known dense-granule (GRA), microneme (MIC), and rhoptry (ROP) genes were not expressed in merozoites. In contrast, a large set of surface proteins (SRS) was expressed exclusively in merozoites. Conclusions The distinct expression profiles of merozoites and tachyzoites reveal significant additional complexity within the T. gondii life cycle, demonstrating that merozoites are distinct asexual dividing stages which are uniquely adapted to their niche and biological purpose.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Toxoplasma gondii
en_US
dc.subject
Apicomplexa
en_US
dc.subject
Coccidia
en_US
dc.subject
Cat
en_US
dc.subject
Enteroepithelial development
en_US
dc.subject
Merozoite
en_US
dc.subject
Schizont
en_US
dc.subject
Comparative transcriptomics
en_US
dc.subject
Surface antigen
en_US
dc.subject
Stage-specific gene expression
en_US
dc.title
Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
BMC Genomics
ethz.journal.volume
16
en_US
ethz.pages.start
66
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004256340
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
ethz.date.deposited
2017-06-11T23:19:54Z
ethz.source
ECIT
ethz.identifier.importid
imp593653fae13ea99153
ethz.ecitpid
pub:172080
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T23:27:09Z
ethz.rosetta.lastUpdated
2018-10-08T16:12:45Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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