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dc.contributor.author
Badertscher, Lukas
dc.contributor.author
Wild, Thomas
dc.contributor.author
Montellese, Christian
dc.contributor.author
Alexander, Leila T.
dc.contributor.author
Bammert, Lukas
dc.contributor.author
Sarazova, Marie
dc.contributor.author
Stebler, Michael
dc.contributor.author
Csucs, Gabor
dc.contributor.author
Mayer, Thomas U.
dc.contributor.author
Zamboni, Nicola
dc.contributor.author
Zemp, Ivo
dc.contributor.author
Horvath, Peter
dc.contributor.author
Kutay, Ulrike
dc.date.accessioned
2018-09-19T15:25:15Z
dc.date.available
2017-06-11T23:37:54Z
dc.date.available
2018-09-19T15:23:49Z
dc.date.available
2018-09-19T15:25:15Z
dc.date.issued
2015-12
dc.identifier.other
10.1016/j.celrep.2015.11.061
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/111511
dc.identifier.doi
10.3929/ethz-b-000111511
dc.description.abstract
Ribosome biogenesis is a highly complex process requiring many assisting factors. Studies in yeast have yielded comprehensive knowledge of the cellular machinery involved in this process. However, many aspects of ribosome synthesis are different in higher eukaryotes, and the global set of mammalian ribosome biogenesis factors remains unexplored. We used an imaging-based, genome-wide RNAi screen to find human proteins involved in 40S ribosomal subunit biogenesis. Our analysis identified ∼300 factors, many part of essential protein modules such as the small subunit (SSU) processome, the eIF3 and chaperonin complexes, and the ubiquitin-proteasome system. We demonstrate a role for the vertebrate-specific factor RBIS in ribosome synthesis, uncover a requirement for the CRL4 E3 ubiquitin ligase in nucleolar ribosome biogenesis, and reveal that intracellular glutamine synthesis supports 40S subunit production.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
ribosome synthesis
en_US
dc.subject
RNAi screening
en_US
dc.subject
cullin E3 ubiquitin ligase
en_US
dc.subject
glutamine metabolism
en_US
dc.title
Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2015-12-17
ethz.journal.title
Cell Reports
ethz.journal.volume
13
en_US
ethz.journal.issue
12
en_US
ethz.pages.start
2879
en_US
ethz.pages.end
2891
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Ribosome synthesis in mammalian cells
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007612702
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03543 - Kutay, Ulrike / Kutay, Ulrike
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02891 - ScopeM / ScopeM
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03543 - Kutay, Ulrike / Kutay, Ulrike
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02891 - ScopeM / ScopeM
ethz.grant.agreementno
144221
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2017-06-11T23:38:24Z
ethz.source
ECIT
ethz.identifier.importid
imp593654069a5ea11442
ethz.ecitpid
pub:172938
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T11:40:41Z
ethz.rosetta.lastUpdated
2018-11-08T02:33:44Z
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true
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