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dc.contributor.author
Hector, Stanton
dc.contributor.author
Willard, Kyle
dc.contributor.author
Bauer, Rolene
dc.contributor.author
Mulako, Inonge
dc.contributor.author
Slabbert, Etienne
dc.contributor.author
Kossmann, Jens
dc.contributor.author
George, Gavin M.
dc.date.accessioned
2018-10-04T13:13:36Z
dc.date.available
2017-06-11T23:38:00Z
dc.date.available
2018-10-04T13:13:36Z
dc.date.issued
2015-12-28
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0145487
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/111522
dc.identifier.doi
10.3929/ethz-b-000111522
dc.description.abstract
Bacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. Dextran, a homoglucan produced by Leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with sugarcane deterioration. A culture-based approach was used to isolate extracellular polysaccharide (EPS) producing bacterial strains from milled sugarcane stalks. Ribosomal RNA sequencing analysis grouped 25 isolates into 4 genera. This study identified 2 bacterial genera not previously associated with EPS production or sucrose degradation. All isolates produced polysaccharide when grown in the presence of sucrose. Monosaccharide analysis of purified polymers by Gas Chromatography revealed 17 EPSs consisting solely of glucose (homoglucans), while the remainder contained traces of mannose or fructose. Dextranase treatment of polysaccharides yielded full digestion profiles for only 11 extracts. Incomplete hydrolysis profiles of the remaining polysaccharides suggest the release of longer oligosaccharides which may interfere with sucrose crystal formation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Diverse Exopolysaccharide Producing Bacteria Isolated from Milled Sugarcane: Implications for Cane Spoilage and Sucrose Yield
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
10
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e0145487
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T23:38:24Z
ethz.source
ECIT
ethz.identifier.importid
imp59365406c79f190348
ethz.ecitpid
pub:172950
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T13:22:02Z
ethz.rosetta.lastUpdated
2021-02-15T01:58:45Z
ethz.rosetta.versionExported
true
ethz.COinS
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