Seamless combination of FACS and microfluidic culturing of cell suspensions using the hanging drop network technology
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Open microfluidic cell culturing devices offer new possibilities to simplify cell loading and culturing due to the fact that liquids and cells are directly accessible. Based on the hanging-drop network concept , we present a seamless work flow for microfluidic cell culturing: The open microfluidic devices are compatible to FACS so that individual cells can be directly sorted into specified culturing compartments in a fully automated way and at high accuracy. Cell culturing is then performed in the hanging drops under controlled perfusion while the overall stability is ensured by applying on-chip drop size control measures. Show more
Book title19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectFACS; cell loading; Microfluidic; Perfusion
Organisational unit03684 - Hierlemann, Andreas / Hierlemann, Andreas
296257 - The Body-on-a-Chip (BoC) (EC)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)
NotesPoster abstract. Poster presentation on October 26, 2015.
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